1001. Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.
A. positive direction
B. 3′ to 5′ direction
C. 5′ to 3′ direction
D. negative direction

1002. The type II restriction endonucleases, bind to DNA sites
A. using H-bonds for specificity
B. that are usually palindromic
C. using non-sequence-dependent backbone interactions
D. all of the above

1003. The enzyme that joins DNA cuts is called
A. joinase
B. ligase
C. DNA phosphorylase
D. reverse transcriptase

1004. Cleavage of DNA by EcoRI endonuclease results in the formation of
A. two 3′-OH ends and two 5′-phosphate ends
B. one 3′-OH end and one 5′-phosphate end
C. two 3′-phosphate ends and two 3′-OH ends
D. one 3′-OH end and two 5′-phosphate end

1005. Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)?
A. Ile and Val
B. Lys and Arg
C. Leu and Ala
D. Cys and Met

1006. Sample retention in the column is measured by
A. retention time
B. retention factor
C. retention index
D. all of these

1007. Column bleeding occurs when
A. elution of the analyte is extended over time
B. the column is cracked and stationary phase leaks out
C. traces of the stationary phase are eluted
D. the column breaks during installation and causes personal injury

1008. Microtubules, motor proteins, and actin filaments are all part of
A. the mechanism of photosynthesis that occurs in chloroplasts
B. the rough ER (endoplasmic reticulum) in prokaryotic cells
C. the cytoskeleton of eukaryotic cells
D. the process that moves small molecules across cell membranes

1009. A certain cell organelle which is made of a double phospholipid bilayer that has many large pores in it, is most likely
A. the nuclear envelope
B. the plasma membrane
C. the mitochondrion
D. the cytoskeleton

1010. What does the Michelson interferometer do?
A. Split a polychromatic beam of radiation into its component wavelengths
B. Selectively filter certain wavelengths from a beam of I.R. radiation
C. Modulate the I.R. signal at a lower frequency, so that it can be observed by a detector
D. none of the above

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